Part:BBa_K2637031:Design

SasA cassette with TDH3 Promoter and ADH1 Terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 366
  Illegal BglII site found at 1593
  Illegal BglII site found at 1662
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

TDH3 Promoter + SasA + ADH1 Terminator. And SasA has been optimized in yeast.

Source

TDH3P and ADH1T are the promoter and terminator of yeast. SasA is from Synechococcus elongatus PCC7942